Electroretinogram Assessment of Retinal Function

Electroretinogram (ERG) was measured using an electrodiagnostic LKC system (UTAS-E 2000; LKC Technologies, Gaithersburg, MD, USA) to assess the function of the retina. Mice were dark adapted overnight before taking measurements for scotopic a-wave and b-wave responses.¹⁰ (link)^,¹⁶ (link) Scotopic rod measurements were recorded following white light flashes of increasing intensities of 0.025, 0.25, and 2.5 log cd·s/m², at time intervals of 10, 20, and 30 seconds. For measurement of photopic cone responses, mice were light adapted to a white background for 10 minutes, followed by flashes of light stimulation at 0, 5, 10, and 25 log cd·s/m² intensities. The trace amplitude between time 0 and the lowest point on the trace was considered the a-wave, and the amplitude between the lowest trace and the highest peak following oscillatory potentials was considered the b-wave.

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Sardar Pasha S.P., Shetty T., Lambert-Cheatham N.A., Sishtla K., Mathew D., Muniyandi A., Patwari N., Bhatwadekar A.D, & Corson T.W. (2021). Retinal Phenotyping of Ferrochelatase Mutant Mice Reveals Protoporphyrin Accumulation and Reduced Neovascular Response. Investigative Ophthalmology & Visual Science, 62(2), 36.

Publication 2021

UTAS-E 2000

Cone Electroretinogram Light Light stimulation Mice Photopic Retina Seconds

Corresponding Organization : Indiana University – Purdue University Indianapolis

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Variable analysis

independent variables

Intensity of white light flashes (0.025, 0.25, and 2.5 log cd·s/m^2)
Intensity of light stimulation (0, 5, 10, and 25 log cd·s/m^2)

dependent variables

Scotopic a-wave and b-wave responses
Photopic cone responses

control variables

Electrodiagnostic LKC system (UTAS-E 2000; LKC Technologies, Gaithersburg, MD, USA) used to measure electroretinogram (ERG)
Mice were dark adapted overnight before taking scotopic measurements
Mice were light adapted to a white background for 10 minutes before taking photopic measurements
Time intervals for scotopic measurements (10, 20, and 30 seconds)

controls

Negative control: Not explicitly mentioned.
Positive control: Not explicitly mentioned.

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