The advanced oxidation protein products (AOPPs) were determined in heart tissue by spectrophotometry. The heart tissue was homogenized and diluted (1:5) in phosphate-buffered saline (PBS; pH 7.4). Next, 200 μL of the sample was added to a 96-well plate together with 10 μL of potassium iodide (1.16 M) and 20 μL of glacial acetic acid. The reading was taken immediately at 340 nm with an ELISA reader (Filter Max F5 Multi-Mode Microplate Readers, Molecular Devices, Silicon Valley, CA, United States). The blank was prepared by replacing the sample with PBS. The AOPP concentrations are expressed as μM of chloramine-T/mg of protein (Valente et al., 2013 (link)).
Free full text: Click here
