Fucoxanthin Modulation of PC12 Cells

Rat pheochromocytoma PC12 cells were purchased from China Center for Type Culture Collection (CTCC, Wuhan, China) and cultured in RPMI1640 medium (Gibco BRL, Grand Island, New York, NY, USA) containing 10% inactivated horse serum (Gibco BRL, Grand Island, New York, NY, USA) supplemented with 10% FBS. PC12 cells were grown in a humidified atmosphere containing 5.0% CO₂ at 37 °C. Fucoxanthin was provided by Biopurify Co., Ltd. (Chengdu, Sichuan, China), for which the purity of Fucoxanthin was 98%, as determined using HPLC detection [40 (link)]. Additionally, Fucoxanthin was dissolved in dimethyl sulfoxide (DMSO, Sigma, St. Louis, MO, USA) and then diluted to desired concentrations.