Cellular immunity was determined in 24 of the initially recruited residents. Individuals were selected to include males (n = 8) and females (n = 16) and with and without prior infection. The selection covers the full range of anti-RBD antibody titers found in the study.
T-cell mediated immune response to SARS-CoV-2 was determined using the T-SPOT SARS-CoV-2 (Oxford Immunotec) according to the manufacturer´s instructions. Immune response to spike protein (S1 Questionnaire) and nucleocapsid after stimulation was measured. Since stimulation to both antigens occurs in separate wells, it should be possible to distinguish between infection and vaccinations. Nil and positive control were included in the assay. The test was considered positive if at least, one stimulation showed 8 or more spots.
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