Cellular Immunity to SARS-CoV-2 in Residents

Cellular immunity was determined in 24 of the initially recruited residents. Individuals were selected to include males (n = 8) and females (n = 16) and with and without prior infection. The selection covers the full range of anti-RBD antibody titers found in the study.
T-cell mediated immune response to SARS-CoV-2 was determined using the T-SPOT SARS-CoV-2 (Oxford Immunotec) according to the manufacturer´s instructions. Immune response to spike protein (S1 Questionnaire) and nucleocapsid after stimulation was measured. Since stimulation to both antigens occurs in separate wells, it should be possible to distinguish between infection and vaccinations. Nil and positive control were included in the assay. The test was considered positive if at least, one stimulation showed 8 or more spots.

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Rodríguez-Avial I., García-Salguero C., Bermejo L., Teja J., Pérez-Cecilia E., Delgado-Iribarren A., Vigara M., Gil P, & Culebras E. (2023). Evolution of antibody titers after two doses of mRNA Pfizer-BioNTech vaccine and effect of the third dose in nursing home residents. PLOS ONE, 18(3), e0282388.

Publication 2023

Anti antibody Antigens Assay Cellular immunity Females Immune response Infection Males Nucleocapsid Sars cov 2 Spike protein Spots T cell Vaccinations

Corresponding Organization : Instituto de Investigación Sanitaria del Hospital Clínico San Carlos

Other organizations : Consejería de Salud y Consumo

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Variable analysis

independent variables

Presence of prior SARS-CoV-2 infection

dependent variables

T-cell mediated immune response to SARS-CoV-2 spike protein
T-cell mediated immune response to SARS-CoV-2 nucleocapsid

control variables

Sex (male and female)
Range of anti-RBD antibody titers

controls

Nil control
Positive control

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