Klebsiella Surveillance in Fattening Pigs

In 2019, caecal samples from fattening pigs sampled at slaughter were included in the NORM-VET surveillance programme [17 ]. Only one pig per herd was included. These samples were available for the current study, and were screened for the presence of Klebsiella spp. Each sample was plated directly onto Simmons citrate agar with 1 % inositol (SCAI; Oxoid) and incubated at 37 °C for 48 h. Presumptive Klebsiella spp. colonies were selected based on morphology, and confirmed as Klebsiella spp. with a MALDI-TOF instrument (Bruker Daltonik).

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Kaspersen H., Franklin-Alming F.V., Hetland M.A., Bernhoff E., Löhr I.H., Jiwakanon J., Urdahl A.M., Leangapichart T, & Sunde M. (2023). Highly conserved composite transposon harbouring aerobactin iuc3 in Klebsiella pneumoniae from pigs. Microbial Genomics, 9(2), mgen000960.

Publication 2023

MALDI-TOF instrument

Agar Caecal Citrate Inositol Klebsiella Maldi Pigs

Corresponding Organization :

Other organizations : Norwegian Veterinary Institute, University of Bergen, Stavanger University Hospital, Khon Kaen University

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Variable analysis

independent variables

None explicitly mentioned

dependent variables

Presence of Klebsiella spp. in caecal samples from fattening pigs

control variables

Only one pig per herd was included
Samples were plated directly onto Simmons citrate agar with 1% inositol (SCAI; Oxoid) and incubated at 37°C for 48h

controls

No positive or negative controls were explicitly mentioned

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