Caco2 Cells Immunofluorescence Imaging

Lab-Tek Chamber Slide w/Cover Glass Slide Sterile (Thermo, Waltham, MA, USA) was used to culture and transfect Caco2 cells, with 100 μL of medium per well. Primary antibodies of SMARCE1 (Abcam, Cambridge, UK, 1:100) and tfec (Santa Cruz, USA, 1:50) were used for double immunofluorescence. The cells were fixed with 4% formalin and incubated with specific primary antibodies overnight at 4°C. Primary antibody binding was visualized using fluorescein-labeled donkey anti-rabbit or anti-mouse IgG (Invitrogen, Carlsbad, CA, USA, 1:500) for 1 h at 25°C. DAPI (Solarbio, Beijing, China) was used for nuclear staining. Images were captured using a confocal laser scanning microscope (Olympus FV1000, Japan).