Protocol detail

Genetically Modified Mouse Strains Protocol

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C57BL/6J (B6) mice were obtained from Japan Slc Inc. (Hamamatsu); B6.SJL-ptprca (B6.SJL, Strain #: 4007) mice congenic at the CD45 locus (CD45.1⁺CD45.2⁻) and Jh^−/− mice (Strain #: 17758) were from Taconic. Il10ra^fl/fl (Strain #: 028146; Liu et al., 2012 (link)), CAG-EGFP (Strain #: 003291), Cd19-Cre (Strain #: 006785), Tlr4^flox/flox (Strain #: 024872), and Rosa26-CreER (Strain #: 008463) mice were from Jackson Laboratories. Il-10 Venus and Il10^fl/fl mice were used as previously reported (Atarashi et al., 2011 (link); Roers et al., 2004 (link)). All mice were maintained in our specific pathogen–free animal facility, and all experiments using mice were approved by the Institutional Animal Care Committee of the Tokyo Medical and Dental University.

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Kanayama M., Izumi Y., Akiyama M., Hayashi T., Atarashi K., Roers A., Sato T, & Ohteki T. (2023). Myeloid-like B cells boost emergency myelopoiesis through IL-10 production during infection. The Journal of Experimental Medicine, 220(4), e20221221.

Publication 2023

C57BL/6J (B6) mice Jh−/− mice CAG-EGFP Cd19-Cre Tlr4flox/flox Rosa26-CreER

Animal Animal care committee Il 10 Mice Specific pathogen free Strain

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Variable analysis

independent variables

Mouse strains obtained from different sources: C57BL/6J (B6) mice, B6.SJL-ptprca (B6.SJL) mice, Jh-/- mice, Il10raf/f mice, CAG-EGFP mice, Cd19-Cre mice, Tlr4flox/flox mice, Rosa26-CreER mice, Il-10 Venus mice, and Il10f/f mice

dependent variables

Unspecified

control variables

All mice were maintained in a specific pathogen-free animal facility
Experiments using mice were approved by the Institutional Animal Care Committee of the Tokyo Medical and Dental University

positive controls

Unspecified

negative controls

Unspecified

Annotations

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