The Citrate test uses a medium in which sodium citrate is the only source of carbon and energy. If an organism can use citrate as the sole source of carbon and energy, it will need to use ammonium salts for nitrogen. This will result in the release of ammonia, causing a color change in the medium from green to blue. Tubes of Simon's citrate agar were each inoculated with a test organism and incubated at 35 0 C for 48 hours. A change in the medium from green to royal blue was recorded as a positive test [16] .
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Citrate Utilization Testing Protocol
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Corresponding Organization :
Other organizations : University of Port Harcourt, Rivers State University
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Variable analysis
independent variables
- Test organism
- Change in the medium from green to royal blue
- Incubation temperature (35 0 C)
- Incubation duration (48 hours)
- Sodium citrate as the sole source of carbon and energy
- Ammonium salts as the source of nitrogen
- Positive control: Not specified
- Negative control: Not specified
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