Wildtype mouse embryonic fibroblasts (MEFs) were harvested from C57BL/6J mice and underwent in vitro electroporation of SBKP, mLama4 SKBP, or mLama4/mItgb1 SBKP plasmids using the Lonza 4D-Nucleofector per the manufacturer’s instructions. Soft agar assays were used to evaluate cell line transformation potential as previously described19 (link). Briefly, 15,000 cells were plated in 0.4% bactoagar RPMI with 10% FBS and penicillin/streptomycin over a 0.6% bactoagar RPMI layer in 6 cm plates. Plates were incubated for 3 weeks to allow colony formation prior to staining with 0.05% crystal violet in 10% ethanol for 1 hour. Colonies were imaged using a Leica inverted light microscope and colonies were manually counted by an investigator blinded to treatment group.
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