Total RNA were isolated from rosette leaves of 6-week-old Arabidopsis plants by using EasyPureTM Plant RNA Kit (Transgene) and following the manufacturer’s protocols.
First strand cDNA was synthesized using 2 μg total RNA by Oligo(dT)-primed reverse transcription using the EazyScript First-Strand DNA Synthesis Super Mix (TransGen Biotech) by following the manufacturer’s instructions. Quantitative RT-PCR (qRT-PCR) was used to examine the expression of chlorophylls synthesis genes, carotenoids synthesis genes, and photosystem structure genes. Arabidopsis gene ACTIN2 (ACT2) were used as a inner control for qRT-PCR. The primers used for qRT-PCR examination of ACT2, MAGNESIUM-PROTOPORPHYRIN IX METHYLTRANSFERASE (CHLM), Mg-chelatase subunit D (CHLD), GERANYLGERANYL PYROPHOSPHATE SYNTHASE 6 (GGPS6), PHYTOENE SYNTHASE (PSY), PHYTOENE DESATURASE (PDS), ZETA-CAROTENE DESATURASE (ZDS), PHOTOSYSTEM I SUBUNIT K (PSAK), and PHOTOSYSTEM I SUBUNIT K (PSAN) have been described previously (Qin et al., 2007 (link); Stephenson and Terry, 2008 (link); Liu et al., 2015 (link)).
The primer pairs used for qRT-PCR examination of CHLOROPHYLL A OXYGENASE (CAO), CHLOROPHYLL SYNTHASE (CHLG), COPPER RESPONSE DEFECT 1 (CRD1), PHOTOSYSTEM I SUBUNIT D-2 (PSAD2) and PHOTOSYSTEM I SUBUNIT E-2 (PSAE2) were listed inTable 1 .
First strand cDNA was synthesized using 2 μg total RNA by Oligo(dT)-primed reverse transcription using the EazyScript First-Strand DNA Synthesis Super Mix (TransGen Biotech) by following the manufacturer’s instructions. Quantitative RT-PCR (qRT-PCR) was used to examine the expression of chlorophylls synthesis genes, carotenoids synthesis genes, and photosystem structure genes. Arabidopsis gene ACTIN2 (ACT2) were used as a inner control for qRT-PCR. The primers used for qRT-PCR examination of ACT2, MAGNESIUM-PROTOPORPHYRIN IX METHYLTRANSFERASE (CHLM), Mg-chelatase subunit D (CHLD), GERANYLGERANYL PYROPHOSPHATE SYNTHASE 6 (GGPS6), PHYTOENE SYNTHASE (PSY), PHYTOENE DESATURASE (PDS), ZETA-CAROTENE DESATURASE (ZDS), PHOTOSYSTEM I SUBUNIT K (PSAK), and PHOTOSYSTEM I SUBUNIT K (PSAN) have been described previously (Qin et al., 2007 (link); Stephenson and Terry, 2008 (link); Liu et al., 2015 (link)).
The primer pairs used for qRT-PCR examination of CHLOROPHYLL A OXYGENASE (CAO), CHLOROPHYLL SYNTHASE (CHLG), COPPER RESPONSE DEFECT 1 (CRD1), PHOTOSYSTEM I SUBUNIT D-2 (PSAD2) and PHOTOSYSTEM I SUBUNIT E-2 (PSAE2) were listed in
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