Immortalized FE1 cells derived from the Muta™ Mouse transgenic rodent model were utilized for particle and metal chloride exposures. These cells retain the characteristics of type I and type II pulmonary alveolar epithelial cells, and have been used in the past to assess the genotoxicity and mutagenicity of both chemicals and NPs [30 (link),31 (link),32 (link),33 (link),34 (link),35 (link)].
Cells were maintained in phenol-red-containing Dulbecco’s Modified Eagle’s Medium Nutrient Mixture: F12 HAM (1:1) culture media (DMEM/F12 (1:1) (1X), Life Technologies, Burlington, ON, Canada) supplemented with 2% fetal bovine serum (FBS, Life Technologies, Burlington, ON, Canada), 1 ng/mL human epidermal growth factor (EGF, Life Technologies, Burlington, ON, Canada), 1 U/mL penicillin G, and 1 µg/mL streptomycin (Life Technologies, Burlington, ON, Canada) in an incubator at 37 °C, with 5% CO2. For CometChip exposures, the same conditions were used, except for the absence of phenol-red in the exposure media.
Cells were maintained in phenol-red-containing Dulbecco’s Modified Eagle’s Medium Nutrient Mixture: F12 HAM (1:1) culture media (DMEM/F12 (1:1) (1X), Life Technologies, Burlington, ON, Canada) supplemented with 2% fetal bovine serum (FBS, Life Technologies, Burlington, ON, Canada), 1 ng/mL human epidermal growth factor (EGF, Life Technologies, Burlington, ON, Canada), 1 U/mL penicillin G, and 1 µg/mL streptomycin (Life Technologies, Burlington, ON, Canada) in an incubator at 37 °C, with 5% CO2. For CometChip exposures, the same conditions were used, except for the absence of phenol-red in the exposure media.
Free full text: Click here
