Matrigel Invasion Chamber Assay

Assay was performed with Matrigel-coated chambers from a BioCoat Matrigel Invasion Chamber Kit (BD Biosciences). Cells with 500 μl in serum-free medium were added into the upper chamber and complete medium was added into the lower chamber. After incubation for 24 h, non-invasive cells in the upper surface of the membrane were removed and the cells invasion to the lower surface of the membrane were fixed. Cell counting was then carried out by photographing the membrane through the microscope [18 (link)] and five random fields were taken.

Free full text: Click here

Wu X.Y., Xu H., Wu Z.F., Chen C., Liu J.Y., Wu G.N., Yao X.Q., Liu F.K., Li G, & Shen L. (2015). Formononetin, a novel FGFR2 inhibitor, potently inhibits angiogenesis and tumor growth in preclinical models. Oncotarget, 6(42), 44563-44578.

Publication 2015

BioCoat Matrigel Invasion Chamber Kit

Assay Cells Matrigel Membrane Microscope Serum

Corresponding Organization :

Other organizations : Nanjing Traditional Chinese Medicine Hospital, Nanjing University, Nanjing Medical University, Jiangsu Province Hospital, Jiangsu Cancer Hospital, Dezhou University

Top 5 similar protocols

Variable analysis

independent variables

Cell type (no specific cell types mentioned)

dependent variables

Invasion of cells to the lower surface of the membrane

control variables

Matrigel-coated chambers from a BioCoat Matrigel Invasion Chamber Kit (BD Biosciences)
Serum-free medium in the upper chamber
Complete medium in the lower chamber
Incubation time (24 hours)

Annotations

Based on most similar protocols

Etiam vel ipsum. Morbi facilisis vestibulum nisl. Praesent cursus laoreet felis. Integer adipiscing pretium orci. Nulla facilisi. Quisque posuere bibendum purus. Nulla quam mauris, cursus eget, convallis ac, molestie non, enim. Aliquam congue. Quisque sagittis nonummy sapien. Proin molestie sem vitae urna. Maecenas lorem.

As authors may omit details in methods from publication, our AI will look for missing critical information across the 5 most similar protocols.

About PubCompare

Our mission is to provide scientists with the largest repository of trustworthy protocols and intelligent analytical tools, thereby offering them extensive information to design robust protocols aimed at minimizing the risk of failures.

We believe that the most crucial aspect is to grant scientists access to a wide range of reliable sources and new useful tools that surpass human capabilities.

However, we trust in allowing scientists to determine how to construct their own protocols based on this information, as they are the experts in their field.

Ready to get started?

Revolutionizing how scientists
search and build protocols!