Genomic Sequencing of T. caerulea Mycelia

Genomic DNA sequencing of T. caerulea was carried out with 50 mg mycelia (10 days, in liquid MEP medium at 25°C with shaking at 100 rpm, in the dark) ground under liquid nitrogen. Genomic DNA was extracted as described above. To prepare a library for Nanopore sequencing, 500 ng DNA was processed with a rapid sequencing kit (Oxford Nanopore Technologies) according to the manufacturer’s instructions and sequenced on a MinION flow cell. The genome was assembled using CANU (62 (link), 63 (link)) v.1.9., based on an expected genome size of 50 Mbp. Signal-level reads were indexed against the draft genome using Nanopolish software (64 (link)). After using minimap (65 (link)) and samtools (66 (link)) to sort and map the reads, a consensus sequence was calculated using Nanopolish (67 (link)). Gene models were individually verified with the Augustus algorithm in Aspergillus mode (68 (link)) and compared with BLAST (69 (link)).

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Lawrinowitz S., Wurlitzer J.M., Weiss D., Arndt H.D., Kothe E., Gressler M, & Hoffmeister D. (2022). Blue Light-Dependent Pre-mRNA Splicing Controls Pigment Biosynthesis in the Mushroom Terana caerulea. Microbiology Spectrum, 10(5), e01065-22.

Publication 2022

rapid sequencing kit

Aspergillus Cell Consensus sequence Genome Library Mycelia Nitrogen

Corresponding Organization :

Other organizations : Friedrich Schiller University Jena, Leibniz-Institut für Naturstoff-Forschung und Infektionsbiologie e. V. - Hans-Knöll-Institut (HKI)

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Variable analysis

independent variables

Mycelia growth conditions (10 days, in liquid MEP medium at 25°C with shaking at 100 rpm, in the dark)

dependent variables

Genomic DNA sequencing of T. caerulea

control variables

Ground mycelia under liquid nitrogen

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