Thermoregulation Monitoring in Otop1 Mice

Littermate wild type and Otop1^-/- mice were implanted intraperitoneally with G2 E-Mitter transponders (Starr Life Sciences, Oakmont, PA) and their T_b, total energy expenditure (TEE), respiratory exchange ratio (RER), and physical activity by beam break were measured using an Oxymax/CLAMS system (Columbus Instruments), as described [16 (link)]. The measurements were performed under different conditions, including ambient temperatures of 8 ˚C, 23 ˚C, 35 ˚C, fasting at 23 ˚C, and CL316243 (0.1 mg/kg, i.p.; ED₅₀ is 0.004–0.008 mg/kg [17 (link)]) at 30 ˚C.

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Tu Y.H., Liu N., Xiao C., Gavrilova O, & Reitman M.L. (2023). Loss of Otopetrin 1 affects thermoregulation during fasting in mice. PLOS ONE, 18(10), e0292610.

Publication 2023

G2 E-Mitter transponders Oxymax/CLAMS system

Cl316243 Clams Energy expenditure Mice Respiratory

Corresponding Organization : National Institutes of Health

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Variable analysis

independent variables

Ambient temperature (8 °C, 23 °C, 35 °C)
Fasting at 23 °C
CL316243 (0.1 mg/kg, i.p.) at 30 °C

dependent variables

Body temperature (Tb)
Total energy expenditure (TEE)
Respiratory exchange ratio (RER)
Physical activity by beam break

control variables

Littermate wild type mice

controls

Littermate wild type mice

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