Yeast One-Hybrid Promoter Analysis

The 2.5 kb sequences upstream of the start codon were used for the promoter analysis. Putative NRE motifs were identified using sequences revised in several recent studies [12 (link),13 (link)] and MAST algorithms available on the MEME suite server (version 5.1.0, https://meme-suite.org/meme/tools/meme (accessed on 1 September 2023)). The transformation of the Saccharomyces cerevisiae strain Y2HGold (Clontech) was performed as described in [38 (link)]. A yeast one-hybrid assay was performed as described in [39 ].

Free full text: Click here

Gancheva M.S, & Lutova L.A. (2023). Nitrogen-Activated CLV3/ESR-Related 4 (CLE4) Regulates Shoot, Root, and Stolon Growth in Potato. Plants, 12(19), 3468.

Publication 2023

Saccharomyces cerevisiae strain Y2HGold

Assay Hybrid Start codon Strain Yeast

Corresponding Organization : St Petersburg University

Top 5 similar protocols

Variable analysis

independent variables

Putative NRE motifs identified using sequences revised in several recent studies [12, 13] and MAST algorithms available on the MEME suite server

dependent variables

Transformation of the Saccharomyces cerevisiae strain Y2HGold (Clontech)
Yeast one-hybrid assay

control variables

The transformation of the Saccharomyces cerevisiae strain Y2HGold (Clontech) was performed as described in [38]

controls

Positive control: Not specified
Negative control: Not specified

Annotations

Based on most similar protocols

Etiam vel ipsum. Morbi facilisis vestibulum nisl. Praesent cursus laoreet felis. Integer adipiscing pretium orci. Nulla facilisi. Quisque posuere bibendum purus. Nulla quam mauris, cursus eget, convallis ac, molestie non, enim. Aliquam congue. Quisque sagittis nonummy sapien. Proin molestie sem vitae urna. Maecenas lorem.

As authors may omit details in methods from publication, our AI will look for missing critical information across the 5 most similar protocols.

About PubCompare

Our mission is to provide scientists with the largest repository of trustworthy protocols and intelligent analytical tools, thereby offering them extensive information to design robust protocols aimed at minimizing the risk of failures.

We believe that the most crucial aspect is to grant scientists access to a wide range of reliable sources and new useful tools that surpass human capabilities.

However, we trust in allowing scientists to determine how to construct their own protocols based on this information, as they are the experts in their field.

Ready to get started?

Revolutionizing how scientists
search and build protocols!