Real-time Cell Migration and Invasion Assay

Migration and invasion assays were performed in 16-well CIM plates in an xCELLigence RTCA DP system (ACEA Biosciences) using matrigel basement membrane matrix (BD) for real-time cell migration analysis as described previously [10 (link)]. Briefly, 30,000 cells per well were seeded as 5 duplicates in serum-free medium at the upper compartment of the CIM plates coated with or without matrigel. Serum-complemented medium was added to the lower compartment of the chamber, and then started measurement in xCELLigence RTCA DP system and analyzed the CI (Cell Index) curves to determine cell invasion activity. For negative controls, we added serum-free medium at both upper and bottom chambers. The concentration of matrigel was 1:6 for KYSE150 cells and 1:10 for KYSE140 cells. The cell index representing the amount of migrated cells was calculated with the RTCA Software from ACEA Biosciences (San Diego, CA). At least three independent experiments were carried out; for each independent experiment, 5 duplicates were performed for each group.

Free full text: Click here

Song B., Cui H., Li Y., Cheng C., Yang B., Wang F., Kong P., Li H., Zhang L., Jia Z., Bi Y., Wang J., Zhou Y., Liu J., Wang J., Zhao Z., Zhang Y., Hu X., Shi R., Yang J., Liu H., Yan T., Li Y., Xu E., Qian Y., Xi Y., Guo S., Chen Y., Wang J., Li G., Liang J., Jia J., Chen X., Guo J., Wang T., Zhang Y., Li Q., Wang C., Cheng X., Zhan Q, & Cui Y. (2015). Mutually exclusive mutations in NOTCH1 and PIK3CA associated with clinical prognosis and chemotherapy responses of esophageal squamous cell carcinoma in China. Oncotarget, 7(3), 3599-3613.

Publication 2015

xCELLigence RTCA DP system matrigel basement membrane matrix RTCA Software

Assays Basement membrane Cell migration Matrigel Rtca Serum

Corresponding Organization :

Other organizations : Shanxi Medical University, First Hospital of Shanxi Medical University, Shanxi Provincial Cancer Hospital, BGI Group (China), Shaanxi Provincial People's Hospital, China Medical University, Chinese Academy of Medical Sciences & Peking Union Medical College

Top 5 similar protocols

Variable analysis

independent variables

Presence or absence of matrigel basement membrane matrix in the upper compartment of the CIM plates

dependent variables

Cell invasion activity measured by the cell index (CI) curves

control variables

Cell seeding density (30,000 cells per well)
Serum-free medium in the upper compartment of the CIM plates
Serum-complemented medium in the lower compartment of the CIM plates
Matrigel concentration (1:6 for KYSE150 cells and 1:10 for KYSE140 cells)

controls

Negative controls: Serum-free medium added to both upper and bottom chambers

Annotations

Based on most similar protocols

Etiam vel ipsum. Morbi facilisis vestibulum nisl. Praesent cursus laoreet felis. Integer adipiscing pretium orci. Nulla facilisi. Quisque posuere bibendum purus. Nulla quam mauris, cursus eget, convallis ac, molestie non, enim. Aliquam congue. Quisque sagittis nonummy sapien. Proin molestie sem vitae urna. Maecenas lorem.

As authors may omit details in methods from publication, our AI will look for missing critical information across the 5 most similar protocols.

About PubCompare

Our mission is to provide scientists with the largest repository of trustworthy protocols and intelligent analytical tools, thereby offering them extensive information to design robust protocols aimed at minimizing the risk of failures.

We believe that the most crucial aspect is to grant scientists access to a wide range of reliable sources and new useful tools that surpass human capabilities.

However, we trust in allowing scientists to determine how to construct their own protocols based on this information, as they are the experts in their field.

Ready to get started?

Revolutionizing how scientists
search and build protocols!