Immunostaining of Sf21 Cells and Larvae

Immunostainings with Sf21 cells and third instar larvae were performed as described previously [47 (link), 48 (link)]. The antibodies used in this study were as follows: primary antibodies: rabbit anti-HA (1:100, Sigma) and mouse anti-Calnexin (1:200, DSHB, 99A-6-2-1); secondary antibodies: goat anti-mouse-Cy3 (1:200, Dianova), goat anti-rabbit-AF633 (1:100, Invitrogen). Confocal images were captured with a laser scanning microscope (Zeiss LSM800). Image processing was done with Fiji and Affinity Photo (Serif).

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Klinke N., Meyer H., Ratnavadivel S., Reinhardt M., Heinisch J.J., Malmendal A., Milting H, & Paululat A. (2022). A Drosophila melanogaster model for TMEM43-related arrhythmogenic right ventricular cardiomyopathy type 5. Cellular and Molecular Life Sciences, 79(8), 444.

Publication 2022

rabbit anti-HA goat anti-mouse-Cy3 LSM800

Antibodies Calnexin Goat Larvae Laser scanning microscope Mouse Rabbit Sf21 cells

Corresponding Organization :

Other organizations : Osnabrück University, Heart and Diabetes Center North Rhine-Westphalia, Roskilde University

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Variable analysis

independent variables

Immunostainings with Sf21 cells
Immunostainings with third instar larvae

dependent variables

Localization and expression of HA-tagged protein
Localization and expression of Calnexin protein

control variables

Primary antibodies: rabbit anti-HA and mouse anti-Calnexin
Secondary antibodies: goat anti-mouse-Cy3 and goat anti-rabbit-AF633
Confocal microscopy imaging using Zeiss LSM800
Image processing using Fiji and Affinity Photo

positive controls

Not explicitly mentioned

negative controls

Not explicitly mentioned

Annotations

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