Naïve State Induction of hESCs

Primed hESCs were routinely cultured with TeSR-E8 (StemCell Technologies) on hESC Qualified Matrigel (Corning). hESCs were reverted to naive state using a one-step induction adapted from published protocols (Guo et al., 2017 (link)). Further details are provided in Supplemental Experimental Procedures.
For consistency, all stem cells used in RNA-seq were cultured in 5% O₂.

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Cinkornpumin J.K., Kwon S.Y., Guo Y., Hossain I., Sirois J., Russett C.S., Tseng H.W., Okae H., Arima T., Duchaine T.F., Liu W, & Pastor W.A. (2020). Naive Human Embryonic Stem Cells Can Give Rise to Cells with a Trophoblast-like Transcriptome and Methylome. Stem Cell Reports, 15(1), 198-213.

Publication 2020

TeSR-E8 hESC Qualified Matrigel

Hescs Matrigel Rna seq Stem cells

Corresponding Organization : McGill University Health Centre

Other organizations : Zhejiang University-University of Edinburgh Institute, Tohoku University, Second Affiliated Hospital of Zhejiang University

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Variable analysis

independent variables

Reversion to naive state using a one-step induction adapted from published protocols (Guo et al., 2017)

dependent variables

RNA-seq (gene expression)

control variables

All stem cells used in RNA-seq were cultured in 5% O2

controls

No positive or negative controls were explicitly mentioned in the provided information.

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