Genomic DNA was extracted from CD19+ cells isolated from transgenic (n=19) and WT spleens (n=2, used as normal hybridization controls). Whole-genome analysis was conducted using a 180-K oligonucleotide mouse aCGH microchip (AMADID 27411, Agilent Technologies), following standard protocols52 (link). Microarray data were extracted and visualized using Feature Extraction v10.7 and Agilent Genomic Workbench v5.0 softwares (Agilent Technologies). Regions with DNA copy number abnormalities were detected using ADM-2 (seta s 6) statistic provided by DNA Analytics, with a minimum number of 5 consecutive probes. Genomic build mm7 was used for the experiment. For comparison of the murine lymphoma genomic changes with those present in human disease, two previously published studies including the aCGH analysis of 136 samples from patients with SMZL were used35 (link)36 (link).
Abnormalities Genomic Human HybridizationLymphoma Microarray Mouse Murine OligonucleotidePatients Transgenic
Corresponding Organization :
Other organizations :
Universidad de Navarra, Centro Nacional de Biotecnología, University of Pecs, University of Cambridge, University of Leicester, MRC Toxicology Unit, Royal Bournemouth Hospital, Clinica Universidad de Navarra, University Hospital Schleswig-Holstein, University of Lübeck, Kiel University, KU Leuven, Centre de Recherche en Cancérologie de Toulouse, Inserm, Centro de Biología Molecular Severo Ochoa, Universidad de Salamanca, Marqués de Valdecilla University Hospital, Hospital San Pedro, Servicio Navarro de Salud, Complejo Hospitalario de Navarra, Hospital del Mar Research Institute, Municipal Institute for Medical Research, Universitat Autònoma de Barcelona, Hospital Universitari Germans Trias i Pujol, Josep Carreras Leukaemia Research Institute, Instituto de Investigación Biomédica de Salamanca, INCLIVA Health Research Institute, Universitat de València, Hospital Clínico Universitario de Valencia, Cornell University
Positive control: WT spleens (n=2) used as normal hybridization controls
Negative control: Not explicitly mentioned
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