Tumors were dissociated as previously described [31 (link)]. Briefly, resected tumors were minced, placed into GentleMACS C tubes (Miltenyi Biotech) with dissociation medium (DMEM with 300 U/ml collagenase IV, 0.1% hyaluronidase, and 2 kU/ml DNase I), processed on the GentleMACS dissociator, rotated (10 rpm; Glas-Col rotator) at 37 °C for 40 min, then processed twice on the GentleMACS dissociator. Remaining debris was removed by passing through a 70-μm mesh. For some samples dead cells were removed by ficoll density gradient centrifugation. Livers were dissociated into a single cell suspension as described by [34 (link)] except Gey’s solution was used to lyse RBC instead of ACK solution. Lungs were dissociated as previously described [35 (link)].