Structural Characterization of Polysaccharides

Gentiobiose, low melting point agarose, and laminarin from Laminaria digitata were purchased from Sigma Aldrich (Germany). Curdlan from Alcaligenes faecalis, lichenan (also known as lichenin) from Icelandic moss, yeast β- glucan, laminaribiose and laminaripentaose were purchased from Megazyme, Ireland. Pustulan from Lasallia pustulata, and porphyran from Porphyra were purchased from Elicityl, France and Carbosynth, United Kingdom, respectively. Used bacterial strains were purchased from the Japan Culture Collection, Riken, Japan.^{1 (link)}H NMR, DEPT135, and 2D NMR (COSY and HSQC) analyses were recorded at the Panjab University, Chandigarh, India.

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Singh R.P., Rajarammohan S., Thakur R, & Hassan M. (2020). Linear and branched β-Glucans degrading enzymes from versatile Bacteroides uniformis JCM 13288T and their roles in cooperation with gut bacteria. Gut Microbes, 12(1), 1826761.

Publication 2020

Gentiobiose low melting point agarose laminarin from Laminaria digitata Curdlan from Alcaligenes faecalis lichenan yeast β- glucan laminaribiose laminaripentaose

Agarose Alcaligenes faecalis Bacterial Curdlan Gentiobiose Laminaria digitata Laminaribiose Laminarin Lasallia pustulata Lichenin Moss Porphyra Porphyran Pustulan Strains Yeast β glucan

Corresponding Organization : National Agri-Food Biotechnology Institute

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Variable analysis

independent variables

Gentiobiose
Low melting point agarose
Laminarin from Laminaria digitata
Curdlan from Alcaligenes faecalis
Lichenan (lichenin) from Icelandic moss
Yeast β-glucan
Laminaribiose
Laminaripentaose
Pustulan from Lasallia pustulata
Porphyran from Porphyra

dependent variables

1H NMR, DEPT135, and 2D NMR (COSY and HSQC) analyses

control variables

Bacterial strains purchased from the Japan Culture Collection, Riken, Japan

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