Flies were cultured on yeast/molasses-based standard fly food (recipe: 10L H2O, 138g agar, 220g molasses, 750g malt extract, 180 dry yeast, 800g corn flour, 100g soy flour, 62.5ml propionic acid, 20g Methyl 4-Hydroxybenzoate, and 72ml ethanol). For GeneSwitch experiments, 86 mg RU486 was additionally dissolved in the ethanol for 200 μM final concentration. Flies were maintained at 25°C with a 12h light/dark cycle and 65% humidity. All flies used in the experiments reported were females.
Fly lines HmlΔ::Gal4, LSP2::Gal4, Elav::Gal4 and W1118 were provided by the Bloomington Drosophila Stock Center. RNAi line MANFRNAiv12834 (UAS::MANFRNAi) was obtained from the Vienna Drosophila RNAi Center. These construct has no predicted off-targets. MANFRNAiv12834 was combined with a transgene driving Dicer overexpression (UAS::DICER2) to achieve efficient MANF knock-down19 (link).
We received the following lines as gifts: UAS::MANF, (T. Heino); NP1::Gal4 (D. Ferrandon); PPL::Gal4 (M. Pankratz); Upd3::Gal4 (N. Perrimon); C7::Gal4 (M. Uhlivora); UAS::Upd2 (M. Zeidler); UAS::Upd3 (N. Buchon); 2xStat::GFP (E. Bach).
Fly lines HmlΔ::Gal4, LSP2::Gal4, Elav::Gal4 and W1118 were provided by the Bloomington Drosophila Stock Center. RNAi line MANFRNAiv12834 (UAS::MANFRNAi) was obtained from the Vienna Drosophila RNAi Center. These construct has no predicted off-targets. MANFRNAiv12834 was combined with a transgene driving Dicer overexpression (UAS::DICER2) to achieve efficient MANF knock-down19 (link).
We received the following lines as gifts: UAS::MANF, (T. Heino); NP1::Gal4 (D. Ferrandon); PPL::Gal4 (M. Pankratz); Upd3::Gal4 (N. Perrimon); C7::Gal4 (M. Uhlivora); UAS::Upd2 (M. Zeidler); UAS::Upd3 (N. Buchon); 2xStat::GFP (E. Bach).
