The β1 integrin gene was isolated from mouse RW4 genomic DNA, and a 5-kb BamHI restriction endonuclease fragment was subcloned and used for preparation of the targeting vector. Electroporations of DNAs into RW4 Agouti ES cells were carried out at 270 V, 500 mF in a GenePulser (Bio-Rad Laboratories). ES cells harboring the desired recombinations were injected into mouse C57BL blastocysts, which were then transferred to CD1 mothers. After breeding, heterozygous and homozygous mice were identified by PCR analysis of toe skin DNAs.
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Targeted Disruption of β1 Integrin in Mice
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Corresponding Organization :
Other organizations : Howard Hughes Medical Institute, University of Chicago
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Variable analysis
independent variables
- Electroporation voltage (270 V)
- Electroporation capacitance (500 mF)
- Successful recombination in RW4 Agouti ES cells
- Identification of heterozygous and homozygous mice by PCR analysis
- Mouse RW4 genomic DNA
- BamHI restriction endonuclease
- RW4 Agouti ES cells
- Mouse C57BL blastocysts
- CD1 mothers
- Positive control: Not explicitly mentioned
- Negative control: Not explicitly mentioned
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