Plasma lipoprotein profile was analyzed by FPLC as described 42 (link). Briefly, after 100 μl plasma was injected, lipoproteins were run at 0.5 ml/min in a buffer containing 0.15 M NaCl, 0.01 M Na2HPO4, 0.1 mM EDTA, pH 7.5, and separated on a Superose 6 10/300 GL column (GE Healthcare) by using BioLogic DuoFlow QuadTec 10 System (Bio-Rad, CA). 500 μl of sample per fraction was collected. Triglyceride or cholesterol levels in each fraction were determined using Infinity reagents (Thermo Fisher).
Protocol detail
Plasma Lipoprotein Profiling by FPLC
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Other organizations : Northeast Ohio Medical University
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Variable analysis
independent variables
- Volume of plasma injected (100 μl)
- Lipoprotein profile
- Triglyceride levels in each fraction
- Cholesterol levels in each fraction
- Buffer composition (0.15 M NaCl, 0.01 M Na2HPO4, 0.1 mM EDTA, pH 7.5)
- Flow rate (0.5 ml/min)
- Column (Superose 6 10/300 GL)
- Separation system (BioLogic DuoFlow QuadTec 10 System)
- No positive or negative controls were explicitly mentioned.
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