Genetic Toolbox for Drosophila Mitochondrial Research

All fly stocks were raised on standard media at 25°C unless otherwise stated. Lines used in this study include nos-Cas9 (BDSC:54591), nubbin-GAL4 (BDSC:42699), nos-GAL4 (BDSC:64308), Ubi-mtSSB-GFP (a gift from Patrick O’Farrell, University of California, San Francisco), Opa1-3HA (Liu et al., 2020 (link)), mt:ND2^del1+CoI^T300I (Ma et al., 2014 (link)), D. melanogaster (mt:mauritiana; Ma and O’Farrell, 2016 (link)), w1118 and w*;attP40. REC-Halo, REC-FLAG, REC^ΔMTS-Halo, REC^KO-Halo, HDM-Halo, PolG1-Halo, XLF1(CG12728)-Halo, SPN-B-Halo, SPN-D-Halo, MRE11-Halo, NBS-Halo, UASp-REC-GFP, UASp-mito-NciI, and mt:NciI^resistant were generated for this paper.

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Klucnika A., Mu P., Jezek J., McCormack M., Di Y., Bradshaw C.R, & Ma H. (2022). REC drives recombination to repair double-strand breaks in animal mtDNA. The Journal of Cell Biology, 222(1), e202201137.

Publication 2022

nubbin-GAL4 nos-GAL4

D melanogaster Mito Opa1

Corresponding Organization : Wellcome/Cancer Research UK Gurdon Institute

Other organizations : South China Agricultural University

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Variable analysis

independent variables

Nos-Cas9
Nubbin-GAL4
Nos-GAL4
Ubi-mtSSB-GFP
Opa1-3HA
Mt:ND2^del1+CoI^T300I
D. melanogaster (mt:mauritiana)
REC-Halo
REC-FLAG
REC^ΔMTS-Halo
REC^KO-Halo
HDM-Halo
PolG1-Halo
XLF1(CG12728)-Halo
SPN-B-Halo
SPN-D-Halo
MRE11-Halo
NBS-Halo
UASp-REC-GFP
UASp-mito-NciI
Mt:NciI^resistant

dependent variables

Not explicitly mentioned

control variables

All fly stocks were raised on standard media at 25°C unless otherwise stated
W1118
W*;attP40

positive controls

Not explicitly mentioned

negative controls

Not explicitly mentioned

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