Protocol detail

Blood RNA Extraction and qPCR Analysis

Find Similar Protocols

Blood RNA was generated using RNeasy protect animal tubes and blood kit (Qiagen). cDNA was synthesized using Qiagen reagents with random hexamers and oligo(dT) primers. Quantitative PCR was performed using SYBR Green reagents (TaKaRa Bio) on a 7500 fast real-time PCR system (Applied Biosystems). Primers were as in (17 (link)).

Partial Protocol Preview
This section provides a glimpse into the protocol.
The remaining content is hidden due to licensing restrictions, but the full text is available at the following link: Access Free Full Text.

Chu T., Ni M., Chen C., Akilesh S, & Hamerman J.A. (2019). BCAP promotes lupus-like disease and TLR-mediated Type I IFN induction in plasmacytoid dendritic cells. Journal of immunology (Baltimore, Md. : 1950), 202(9), 2529-2534.

Publication 2019

SYBR Green reagents 7500 fast real-time PCR system

Animal Blood Cdna Oligo Primers Sybr green

Top 5 similar protocols

Variable analysis

independent variables

RNA extraction method (RNeasy protect animal tubes and blood kit from Qiagen)
CDNA synthesis reagents (Qiagen reagents with random hexamers and oligo(dT) primers)
QPCR reagents (SYBR Green from TaKaRa Bio)
QPCR platform (7500 fast real-time PCR system from Applied Biosystems)
Primers used (as in reference 17)

dependent variables

Gene expression levels measured by quantitative PCR

control variables

Not explicitly mentioned

positive controls

Not specified

negative controls

Not specified

Annotations

Based on most similar protocols

Etiam vel ipsum. Morbi facilisis vestibulum nisl. Praesent cursus laoreet felis. Integer adipiscing pretium orci. Nulla facilisi. Quisque posuere bibendum purus. Nulla quam mauris, cursus eget, convallis ac, molestie non, enim. Aliquam congue. Quisque sagittis nonummy sapien. Proin molestie sem vitae urna. Maecenas lorem.

As authors may omit details in methods from publication, our AI will look for missing critical information across the 5 most similar protocols.

About PubCompare

Our mission is to provide scientists with the largest repository of trustworthy protocols and intelligent analytical tools, thereby offering them extensive information to design robust protocols aimed at minimizing the risk of failures.

We believe that the most crucial aspect is to grant scientists access to a wide range of reliable sources and new useful tools that surpass human capabilities.

However, we trust in allowing scientists to determine how to construct their own protocols based on this information, as they are the experts in their field.

Ready to get started?

Revolutionizing how scientists
search and build protocols!