Quantifying Urease and Hydrogen Uptake in H. pylori

Urease activity of whole H. pylori cells was assayed by measuring the ammonia production using the Ammonia-Assay kit (Sigma) as described [13 (link)]. The NH₃ concentration in the supernatant was measured with the Ammonia-Assay kit according to the manufacturer’s (Sigma) instructions. Hydrogen uptake activity was determined spectrophotometrically at 604 nm by following the color change of methyl viologen (MV) from a colorless oxidized form to a dark-violet reduced form as described in [20 (link)]. The data correspond to at least three independent experiments with two technical replicates each time.

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Denic M., Turlin E., Michel V., Fischer F., Khorasani-Motlagh M., Zamble D., Vinella D, & de Reuse H. (2021). A novel mode of control of nickel uptake by a multifunctional metallochaperone. PLoS Pathogens, 17(1), e1009193.

Publication 2021

Ammonia-Assay kit

Ammonia Assay Cells H pylori Hydrogen Methyl viologen Urease Violet

Corresponding Organization : Centre National de la Recherche Scientifique

Other organizations : Université Paris Cité, Sorbonne Paris Cité, Université de Strasbourg, Génétique Moléculaire Génomique Microbiologie, University of Toronto

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Variable analysis

independent variables

Urease activity of whole H. pylori cells
Hydrogen uptake activity

dependent variables

Ammonia production
Color change of methyl viologen (MV) from a colorless oxidized form to a dark-violet reduced form

control variables

Not explicitly mentioned

controls

Positive control: Not specified
Negative control: Not specified

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