The acetylcholinesterase activities of the extracts of M. myrtifolia were measured with the AChE activity of Electrophorus electricus (electric eels) spectrophotometrically using the Ellman Method [23] (link). The substrate used in the study was acetylthiocholine iodide (ATC). The reactions were carried out at 25°C, in 100 mM Tris HCl (pH 8.0) buffer. To start each enzymatic reaction approximately 0.05 U/ml AChE was added to the reaction mixture. The breakdown of acetylthiocholine was detected with a UV spectrophotometer (Carry 60 Single Beam spectrophotometer, Agilent Technologies, USA) over an elevation in absorbance at 412 nm.
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Acetylcholinesterase Activity Assay
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Other organizations : Altınbaş University
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Variable analysis
independent variables
- Extracts of M. myrtifolia
- Acetylcholinesterase (AChE) activities of the extracts
- Reaction temperature (25°C)
- Tris HCl buffer (100 mM, pH 8.0)
- Approximately 0.05 U/ml AChE added to start the enzymatic reaction
- Acetylthiocholine iodide (ATC) used as the substrate
- Electrophorus electricus (electric eels) AChE activity as a positive control
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