Immunohistochemical Analysis of Glioma

The expression of HACE1, NRF2, and Ki-67 in glioma tissues was evaluated by IHC using HACE1 antibody (Abcam), NRF2 antibody (Santa Cruz Biotechnology, Inc.), and Ki-67 antibody (BD Pharmingen). The detailed protocol was similarly described according to a previous study.³⁸ Sections were then analyzed by a Tissue FAXS system (Tissuegnostics USA, Tarzana, CA, USA), and positive cells were directly counted using HistoQuest cytometry software according to the principle of flow cytometry.^{39 (link)}

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Da C., Pu J., Liu Z., Wei J., Qu Y., Wu Y., Shi B., Yang J., He N, & Hou P. (2021). HACE1-mediated NRF2 activation causes enhanced malignant phenotypes and decreased radiosensitivity of glioma cells. Signal Transduction and Targeted Therapy, 6, 399.

Publication 2021

NRF2 antibody Ki-67 antibody Tissue FAXS system

Antibody Cells Flow cytometry Glioma Nrf2 Tissue

Corresponding Organization : Southeast University

Other organizations : First Affiliated Hospital of Xi'an Jiaotong University

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Variable analysis

independent variables

HACE1 expression
NRF2 expression
Ki-67 expression

dependent variables

Expression of HACE1, NRF2, and Ki-67 in glioma tissues

control variables

Use of HACE1 antibody (Abcam)
Use of NRF2 antibody (Santa Cruz Biotechnology, Inc.)
Use of Ki-67 antibody (BD Pharmingen)
Analysis using Tissue FAXS system (Tissuegnostics USA, Tarzana, CA, USA)
Analysis using HistoQuest cytometry software

controls

Positive control: Not explicitly mentioned.
Negative control: Not explicitly mentioned.

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