Quantifying Blood SNO Levels

To determine SNO-Hb levels, blood samples (~ 2 mL) were drawn from the subject’s radial artery, prior to any ischemia intervention. RBC SNO levels were quantified offline using photolysis-chemiluminescence (39 (link)). Additional analyses (nitrite, nitrate, blood gasses) were conducted by the research team or by University Hospital’s Clinical Chemistry laboratory (complete blood count, complete metabolic panel).

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Reynolds J.D., Posina K., Zhu L., Jenkins T., Matto F., Hausladen A., Kashyap V., Schilz R., Zhang R., Mannick J., Klickstein L., Premont R.T, & Stamler J.S. (2023). Control of tissue oxygenation by S-nitrosohemoglobin in human subjects. Proceedings of the National Academy of Sciences of the United States of America, 120(9), e2220769120.

Publication 2023

Blood Chemiluminescence Clinical laboratory Complete blood count Gasses Ischemia Nitrate Nitrite Photolysis Radial artery

Corresponding Organization :

Other organizations : University Hospitals of Cleveland, Case Western Reserve University, Discovery Institute

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Variable analysis

independent variables

Ischemia intervention

dependent variables

RBC SNO levels

control variables

Blood sample (~ 2 mL) drawn from the subject's radial artery, prior to any ischemia intervention

controls

No positive or negative controls were explicitly mentioned in the input protocol.

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