Subgroup of mice received various interventions. We intraperitoneally injected either tauroursodeoxycholic acid (TUDCA) (150 mg/kg bodyweight, dissolved in saline) or saline once daily starting 18 weeks after the last streptozotocin (STZ) injection until 1 d before analyses (week 26) in some mice5 (link). In other mice we intraperitoneally injected aPC (1 mg/kg bodyweight, every alternate day) or an equal volume of saline. Aditionally, in a subgroup of mice aPC was pre-incubated before injection with the HAPC1573 antibody at a 1:1 ratio for 10 min under gentle agitation to block its anticoagulant activity20 (link),21 (link). Human aPC was used throughout the study, which was generated following an established protocol22 (link).
Streptozotocin-Induced Diabetes Protocol
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Corresponding Organization : Johannes Gutenberg University Mainz
Other organizations : Huazhong University of Science and Technology, Tongji Hospital, Vanderbilt University Medical Center, Istanbul Bilim University, University of Sargodha, Helmholtz Centre for Infection Research, Oklahoma Medical Research Foundation, Rush University, TU Dresden, Scripps Research Institute
Protocol cited in 3 other protocols
Variable analysis
- Low dose streptozotocin (STZ, 60 mg/kg, freshly dissolved in 0.05 M sterile sodium citrate, pH 4.5) administered on five successive days
- Tauroursodeoxycholic acid (TUDCA) (150 mg/kg bodyweight, dissolved in saline) injected intraperitoneally once daily
- Activated protein C (aPC) (1 mg/kg bodyweight) injected intraperitoneally every alternate day
- APC pre-incubated with the HAPC1573 antibody at a 1:1 ratio for 10 min under gentle agitation before injection
- Insulinopenia
- Persistent hyperglycaemia
- Consequences of insulin resistance
- Saline used as a control for TUDCA and aPC injections
- 8-week-old mice used
- Db/db mice used to study insulin resistance
- None specified
- Saline injections used as a control for TUDCA and aPC treatments
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