Genetic Analyses of Melioidosis Patients in Thailand

Human genetic analyses were performed on patients with culture-proven melioidosis admitted to Sappasithiprasong Hospital, Ubon Ratchathani, Thailand from 1999 through 2005 [8] (link)–[10] (link). A study team screening patients with clinical signs of infection cultured sputum, blood, urine and other relevant samples (for example, abscess aspirates) for B. pseudomallei. Samples from some patients were independently submitted for culture by hospital clinicians. Pulmonary involvement was defined by a positive B. pseudomallei culture from the respiratory tract or radiographic evidence of a pulmonary infiltrate and a positive B. pseudomallei culture from another clinical specimen. Deaths were defined as those individuals who died during their hospitalization or were discharged home in extremis for palliative care. DNA was extracted from blood using a Nucleon BACC3 kit (GE Healthcare, Buckinghamshire, UK).

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West T.E., Myers N.D., Chantratita N., Chierakul W., Limmathurotsakul D., Wuthiekanun V., Miao E.A., Hajjar A.M., Peacock S.J., Liggitt H.D, & Skerrett S.J. (2014). NLRC4 and TLR5 Each Contribute to Host Defense in Respiratory Melioidosis. PLoS Neglected Tropical Diseases, 8(9), e3178.

Publication 2014

Nucleon BACC3 kit

Abscess Blood Hospitalization Infection Melioidosis Patients Pulmonary Radiographic Respiratory tract Sputum Urine

Corresponding Organization : University of Cambridge

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Variable analysis

independent variables

None explicitly mentioned

dependent variables

Development of culture-proven melioidosis
Pulmonary involvement (defined by positive B. pseudomallei culture from respiratory tract or radiographic evidence of pulmonary infiltrate and positive B. pseudomallei culture from another clinical specimen)
Mortality (defined as individuals who died during hospitalization or were discharged home in extremis for palliative care)

control variables

None explicitly mentioned

controls

No positive or negative controls were explicitly mentioned in the provided information.

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