Histological Evaluation of Liver Injury

Formalin-fixed paraffin-embedded liver sections (5μm) were stained with hematoxylin and eosin. The severity of IRI was graded using Suzuki’s criteria.^{21 (link)}The mouse liver infiltrating macrophages and neutrophils were detected with rat anti-CD11b Ab (BD Biosciences, San Jose, CA) and rat anti-Ly6G Ab (Bio-Rad, Hercules, CA), respectively. Signals were visualized with secondary Alexa Fluor 488 anti-rat IgG. Results were scored semi-quantitatively by blindly counting the number of positive cells in 10 HPF/section (x400).

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Kageyama S., Kadono K., Hirao H., Nakamura K., Ito T., Gjertson D.W., Sosa R.A., Reed E.F., Kaldas F.M., Busuttil R.W., Kupiec-Weglinski J.W, & Zhai Y. (2021). Ischemia-Reperfusion Injury in Allogeneic Liver Transplantation: A Role of CD4 T Cells in Early Allograft Injury. Transplantation, 105(9), 1989-1997.

Publication 2020

rat anti-CD11b Ab rat anti-Ly6G Ab

Alexa fluor 488 Anti igg Cd11b Eosin Formalin Liver Liver macrophages Mouse Neutrophils Paraffin

Corresponding Organization : University of California, Los Angeles

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Variable analysis

independent variables

Formalin-fixed paraffin-embedded liver sections (5μm)

dependent variables

Severity of IRI graded using Suzuki's criteria
Number of mouse liver infiltrating macrophages and neutrophils

control variables

Hematoxylin and eosin staining
Rat anti-CD11b Ab and rat anti-Ly6G Ab used to detect macrophages and neutrophils, respectively
Alexa Fluor 488 anti-rat IgG used to visualize signals
Positive and negative controls not explicitly mentioned

Annotations

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