Cell culture conditions are described in Supplemental Materials and Methods . Transient transfections were performed using miScript miR‐26a‐5p mimetic, inhibitor or control (Qiagen) or at 20 or 50 nM and Lipofectamine 2000 (Life Technologies, Carlsbad, CA) in human embryonic kidney (HEK293), HOK, PDL‐F and HeLa cells as previously described (Naqvi et al., 2018a (link), 2018b (link)). Red siGLO oligos (ThermoScientific) were used as transfection controls for every experiment. Transfection efficiency data are shown in Figure S1 .
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Transient Transfection of miR-26a-5p in Cell Lines
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Corresponding Organization : University of Illinois Chicago
Other organizations : University of Iowa, Universidad Autónoma de Nuevo León, University of Puerto Rico System
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Variable analysis
independent variables
- MiScript miR‐26a‐5p mimetic, inhibitor or control (Qiagen) at 20 or 50 nM
- Not explicitly mentioned
- Cell culture conditions (described in Supplemental Materials and Methods)
- Lipofectamine 2000 (Life Technologies, Carlsbad, CA) for transfections
- Red siGLO oligos (ThermoScientific) as transfection controls
- Positive control: Red siGLO oligos (ThermoScientific) as transfection controls
- Negative control: miScript miR‐26a‐5p control (Qiagen)
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