The sample used in the metabolomic analysis was the same as the sample used for RNA‐seq. Metabolite extraction was conducted according to the manufacturer's instruction (Wuhan MetWare Biotechnology Co., Ltd., Wuhan, China), and metabolomic analysis was carried out using UPLC‐MS/MS (UPLC: Shim‐pack UFLC SHIMADZU CBM30A, Kyoto, Japan; MS/MS: Applied Biosystems 4500 Q TRAP, Foster City, CA, USA) according to the method described in our previous study (Ding et al., 2019a (link)). Three independent biological replications were performed. The identified metabolites were annotated using the metware database (http://www.metware.cn ). Differential metabolites between groups were determined by ¦Log2FC¦ ≥ 1 and VIP ≥1.
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Metabolomic Analysis of Biological Samples
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Biological
Ms ms
Replications
Rna seq
Shim
Corresponding Organization :
Other organizations : Ministry of Agriculture and Rural Affairs, Nanjing Agricultural University
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Variable analysis
independent variables
- Metabolite extraction method
- Metabolomic profile
- Differential metabolites between groups
- Biological sample used for both metabolomic analysis and RNA-seq
- Metabolite extraction protocol
- UPLC-MS/MS analysis method
- Three independent biological replications were performed
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