Retinoids Analysis by HPLC

Retinoids were analyzed by normal-phase HPLC as described previously (38 (link)). In brief, retinoids in hexane extractions were evaporated, redissolved in 100 μl of hexane, and separated on a silica column (Zorbax-Sil, 5 μm, 250 × 4.6 mm, Agilent Technologies) by gradient (0.2–10% dioxane in hexane at 2.0 ml/min flow rate) or non-gradient (10% dioxane in hexane at 1.0 ml/min flow rate) elution of the mobile phase in an Agilent 1100 liquid chromatograph.

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Li S., Izumi T., Hu J., Jin H.H., Siddiqui A.A., Jacobson S.G., Bok D, & Jin M. (2014). Rescue of Enzymatic Function for Disease-associated RPE65 Proteins Containing Various Missense Mutations in Non-active Sites. The Journal of Biological Chemistry, 289(27), 18943-18956.

Publication 2014

Zorbax-Sil Agilent 1100 liquid chromatograph

Dioxane Hexane Hplc Liquid chromatograph Retinoids Silica

Corresponding Organization :

Other organizations : Louisiana State University Health Sciences Center New Orleans, University of Kentucky, University of California, Los Angeles, Doheny Eye Institute, Washington University in St. Louis, Tulane University, University of Pennsylvania

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Variable analysis

independent variables

Gradient elution of the mobile phase (0.2–10% dioxane in hexane at 2.0 ml/min flow rate)
Non-gradient elution of the mobile phase (10% dioxane in hexane at 1.0 ml/min flow rate)

dependent variables

Separation of retinoids

control variables

Silica column (Zorbax-Sil, 5 μm, 250 × 4.6 mm, Agilent Technologies)
Agilent 1100 liquid chromatograph

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