Regulation of EGFR Signaling by miR-203
Corresponding Organization :
Other organizations : Taipei Medical University, Wan Fang Hospital, American University of Beirut, National Cancer Institute, National Institutes of Health, Taipei Medical University-Shuang Ho Hospital, University of California, Los Angeles
Protocol cited in 5 other protocols
Variable analysis
- EGF (from R&D Systems, MN)
- EGFR inhibitor CI1033 (from Selleck, TX)
- EGFR inhibitor AG1478 (from Selleck, TX)
- Empty vector and miR-203 precursor (from GeneCopoeia, MD)
- Control and anti-miR-203 (from GeneCopoeia, MD)
- Invasion assay
- Tumor cell injections
- BD Matrigelâ„¢ (from BD Biosciences, CA)
- RFP reporter vectors (constructed using the Clone-it Enzyme free Lenti-vectors Kit from System Biosciences, CA)
- Human AREG, EREG, and TGFA full length 3'UTR reporters (constructed using the psiHECKTM-2 vector from Promega, WI)
- MicroRNA binding site mutations (made using the Site-Directed Mutagenesis System kit from Invitrogen, CA)
- No positive controls explicitly mentioned.
- Empty vector
- Control anti-miR
Annotations
Based on most similar protocols
As authors may omit details in methods from publication, our AI will look for missing critical information across the 5 most similar protocols.
About PubCompare
Our mission is to provide scientists with the largest repository of trustworthy protocols and intelligent analytical tools, thereby offering them extensive information to design robust protocols aimed at minimizing the risk of failures.
We believe that the most crucial aspect is to grant scientists access to a wide range of reliable sources and new useful tools that surpass human capabilities.
However, we trust in allowing scientists to determine how to construct their own protocols based on this information, as they are the experts in their field.
Ready to get started?
Sign up for free.
Registration takes 20 seconds.
Available from any computer
No download required
Revolutionizing how scientists
search and build protocols!