Whole cell lysates were prepared, resolved and blotted as described previously [20 (link)]. CXCR4 expression was detected using an anti-human CXCR4 antibody (Abcam) at 1:1000. Expression of CXCR1 and CXCR2 were detected as previously described [30 (link)]. Membranes were re-probed with GAPDH antibody to ensure equal loading (Biogenesis, Dorset, UK).
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