Western Blot Analysis of Apoptosis Markers

Samples were prepared as described previously [26 (link)]. Anti BCL2 (1:500 dilution) and anti MCL1 Abs (1:250) were purchased from Becton Dickinson. Abs against the phosphorylated and unphosphorylated forms of p38 and MAP2K3 (1:10,000 each) and against BCL6 (1:1,000), BCLXL (1:1,000) and MYC (1:2,000) were obtained from Cell Signaling Technology (Leiden, The Netherlands), the GAPDH mouse monoclonal Ab (mAb) (1:5,000) was from Abcam (Cambridge, UK). Specific bands on nitrocellulose membranes were visualized with the biotin/streptavidin-horseradish peroxidase system (GE Healthcare, Little Chalfont, UK) in combination with the “Renaissance Western Blot Chemoluminescence Reagent” protocol (Perkin Elmer, Waltham, MA, USA).