Protocol detail

Evaluating Cytotoxicity of CeO2 NPs in ARPE-19 Cells

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Cell Titer-Glo^® Luminescent Cell Viability Assay (Promega, Madison, WI, USA) was used to assess the ATP levels in CeO₂ NPs-treated ARPE-19 cells according to the manufacturer’s instructions. Luminescence was recorded using a Synergy H4 Hybrid microplate reader.
The cytotoxicity of CeO₂ NPs was examined using the LDH Release Assay (Beyotime, Beijing, China) as described previously.27 (link)

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Ma Y., Li P., Zhao L., Liu J., Yu J., Huang Y., Zhu Y., Li Z., Zhao R., Hua S., Zhu Y, & Zhang Z. (2021). Size-Dependent Cytotoxicity and Reactive Oxygen Species of Cerium Oxide Nanoparticles in Human Retinal Pigment Epithelia Cells. International Journal of Nanomedicine, 16, 5333-5341.

Publication 2021

Cell Titer-Glo® Luminescent Cell Viability Assay LDH Release Assay

Assay Cytotoxicity Hybrid Luminescence Luminescent assay Promega

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Variable analysis

independent variables

CeO2 NPs

dependent variables

ATP levels in CeO2 NPs-treated ARPE-19 cells
Cytotoxicity of CeO2 NPs

control variables

Not explicitly mentioned

controls

Positive control: Not specified
Negative control: Not specified

Annotations

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