Cultivation and Mutant Generation of B. pertussis

B. pertussis strain Tohama I was maintained in the laboratory. A dnt-deficient mutant of B. pertussis Tohama I was generated according to methods described previously (45 (link)). B. pertussis was grown in Stainer-Scholte (SS) medium or on Bordet-Gengou agar (Becton, Dickinson, Franklin Lakes, NJ, USA) containing 0.4% (wt/vol) polypeptone or Hipolypeptone (Wako Pure Chemical Industries, Ltd., Japan), 0.8% glycerol, 20% defibrinated horse blood, and 10 μg/ml ceftibuten (BG plate). The culture supernatants of B. pertussis were harvested by centrifugation at 6,800 × g for 5 min and filtered through a 0.22-μm-pore-size filter.

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Teruya S., Hiramatsu Y., Nakamura K., Fukui-Miyazaki A., Tsukamoto K., Shinoda N., Motooka D., Nakamura S., Ishigaki K., Shinzawa N., Nishida T., Sugihara F., Maeda Y, & Horiguchi Y. (2020). Bordetella Dermonecrotic Toxin Is a Neurotropic Virulence Factor That Uses CaV3.1 as the Cell Surface Receptor. mBio, 11(2), e03146-19.

Publication 2020

Bordet-Gengou agar polypeptone HiPolypeptone

Agar B pertussis Blood Ceftibuten Centrifugation G 800 Glycerol Horse Pertussis Size

Corresponding Organization :

Other organizations : Osaka University, Fujita Health University

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Variable analysis

independent variables

B. pertussis strain Tohama I
dnt-deficient mutant of B. pertussis Tohama I

dependent variables

Not explicitly mentioned

control variables

Growth conditions (Stainer-Scholte (SS) medium or Bordet-Gengou agar with 0.4% (wt/vol) polypeptone or HiPolypeptone, 0.8% glycerol, 20% defibrinated horse blood, and 10 μg/ml ceftibuten)
Culture supernatant harvesting (centrifugation at 6,800 × g for 5 min and filtering through a 0.22-μm-pore-size filter)

controls

Positive control: not mentioned
Negative control: not mentioned

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