Ultrahigh-Performance Liquid Chromatography-Tandem Mass Spectrometry for Targeted Quantification

Ultrahigh-performance liquid chromatography–tandem mass spectrometer targeted quantitative analysis specific operation steps refer to our previous report (15 (link)). The UHPLC separation was carried out using an Agilent 1290 Infinity II series UHPLC System (Agilent Technologies, California, USA), equipped with a Waters ACQUITY UPLC BEH Amide column (Waters Corporation, USA) (100 × 2.1 mm, 1.7 μm). Mobile phase A was 1% formic acid in water, and mobile phase B was 1% formic acid in acetonitrile. The column temperature and auto-sampler temperature were set at 35 and 4°C, respectively. An AJS–electrospray ionization interface was connected with Agilent 6460 triple quadrupole mass spectrometer (Agilent Technologies) for assay development. The MRM parameters of the target analytes are controlled by flowing injection of the standard solution of a single analyte.