As previously described (Rao et al., 2014 (link)), 1 μg of total RNA for each sample was used to construct an RNA sequencing (RNA-seq) library using TruSeq RNA Sample Prep Kits v2 (Illumina Inc., San Diego, CA, USA), according to the manufacturer’s instructions, at the Genomics Core Facility at the Noble Foundation (Ardmore, OK, USA). Each library was indexed. Six libraries with different indexes were pooled together in one lane for 100bp paired-end sequencing. The Hiseq2000 run was conducted at the Genomics Core Facility of the Oklahoma Medical Research Foundation (Oklahoma City, OK, USA).
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