For ex vivo pituitary culture, anterior pituitaries were isolated from Hesx1Cre/+;Ctnnb1lox(ex3)/+ embryos at 18.5 dpc, placed on top of 0.2 µM Whatman filters (SLS) in 24 well plates containing 500 µl of media (DMEM-F12 (Gibco), 1% Pen/Strep (Sigma) and 1% FBS (PAA) supplemented with either ABT-737 at 2.5 µM (Adooq Bioscience), ABT-263 at 1 µM (Caymen Chemical) or vehicle. Anterior pituitaries were cultured for 3–4 days, changing media every day and subsequently processed for histological analysis.
Other organizations :
University College London, Great Ormond Street Hospital, Imperial College London, Cancer Genetics (United States), Ludwig Cancer Research, University of Oxford, King's College London, Guy's Hospital
Anterior pituitaries isolated from Hesx1Cre/+;Ctnnb1lox(ex3)/+ embryos at 18.5 dpc
Anterior pituitaries placed on top of 0.2 µM Whatman filters in 24 well plates
Media: DMEM-F12, 1% Pen/Strep, 1% FBS
Anterior pituitaries cultured for 3-4 days, changing media every day
controls
Positive control: Not specified
Negative control: Vehicle
Annotations
Based on most similar protocols
Etiam vel ipsum. Morbi facilisis vestibulum nisl. Praesent cursus laoreet felis. Integer adipiscing pretium orci. Nulla facilisi. Quisque posuere bibendum purus. Nulla quam mauris, cursus eget, convallis ac, molestie non, enim. Aliquam congue. Quisque sagittis nonummy sapien. Proin molestie sem vitae urna. Maecenas lorem.
As authors may omit details in methods from publication, our AI will look for missing critical information across the 5 most similar protocols.
About PubCompare
Our mission is to provide scientists with the largest repository of trustworthy protocols and intelligent analytical tools, thereby offering them extensive information to design robust protocols aimed at minimizing the risk of failures.
We believe that the most crucial aspect is to grant scientists access to a wide range of reliable sources and new useful tools that surpass human capabilities.
However, we trust in allowing scientists to determine how to construct their own protocols based on this information, as they are the experts in their field.
Ready to
get started?
Sign up for free.
Registration takes 20 seconds.
Available from any computer
No download required
