Neprilysin Activity in Mouse Plasma

Lateral saphenous vein blood from conscious, non-fasted mice was collected following eight weeks of treatment. Plasma neprilysin activity was assessed by a fluorometric enzyme method, as previously described (11 (link)). Each plasma sample was assayed in both the absence and presence of a specific neprilysin inhibitor (DL-thiorphan, Sigma-Aldrich, St Louis, MO, USA) to differentiate neprilysin activity from non-specific endopeptidase activity.

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Esser N., Schmidt C., Barrow B.M., Cronic L., Hackney D.J., Mongovin S.M., Hogan M.F., Templin A.T., Castillo J.J., Hull R.L, & Zraika S. (2022). Insulinotropic Effects of Neprilysin and/or Angiotensin Receptor Inhibition in Mice. Frontiers in Endocrinology, 13, 888867.

Publication 2022

DL-thiorphan

Blood Conscious Endopeptidase Enzyme Fluorometric Mice Neprilysin Plasma Saphenous vein Thiorphan

Corresponding Organization : VA Puget Sound Health Care System

Other organizations : University of Liège

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Variable analysis

independent variables

Duration of treatment (eight weeks)

dependent variables

Plasma neprilysin activity

control variables

Conscious, non-fasted mice
Lateral saphenous vein blood collection

controls

Positive control: Plasma samples assayed in the presence of a specific neprilysin inhibitor (DL-thiorphan) to differentiate neprilysin activity from non-specific endopeptidase activity
Negative control: Not explicitly mentioned

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