Transmembrane Cell Migration Assay

was performed as we described previously [49 (link)] with few minor modifications. 1.5 x 10⁶ cells/ml were seeded on top of the gelatinized microporous membrane (8μm pore size, 6.5 mm Corning insert). After two hours, serum-free media was added to the bottom part of the well and incubated at 37°C overnight. Migrated cells were then fixed and stained using the Diff-Quick staining kit (Dade Behring, Deerfield, IL) and counted in 10 high-powered fields (100X).

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Sennoune S.R., Nelius T., Jarvis C., Pruitt K., Kottapalli K.R, & Filleur S. (2020). The Wnt non-canonical signaling modulates cabazitaxel sensitivity in prostate cancer cells. PLoS ONE, 15(6), e0234078.

Publication 2020

Diff-Quick staining kit

Cells Dade Membrane Serum free media

Corresponding Organization : American University of Antigua

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Variable analysis

independent variables

Seeding density (1.5 x 10^6 cells/ml)

dependent variables

Number of migrated cells

control variables

Gelatinized microporous membrane (8μm pore size, 6.5 mm Corning insert)
Serum-free media added to the bottom part of the well
Incubation at 37°C overnight
Diff-Quick staining kit (Dade Behring, Deerfield, IL)

controls

Positive control: Not mentioned
Negative control: Not mentioned

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