Calibrating Xenon Light Intensity

A xenon high-pressure lamp (Lambda LS, Sutter Instruments) was used, and the light stimuli were delivered to the ommatidia by means of epi-illumination via the objective lens (in situ). The effective intensity of the orange light (Schott OG 590 edge filter) at the specimen was calibrated using a bioassay, by measuring the rate of single photon responses (bumps) from WT flies at normal Ca²⁺ conditions (effective photons per second, as previously described (36 (link))).

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Gutorov R., Katz B., Peters M, & Minke B. (2023). Membrane lipid modulations by methyl-β-cyclodextrin uncouple the Drosophila light-activated phospholipase C from TRP and TRPL channel gating. The Journal of Biological Chemistry, 300(1), 105484.

Publication 2023

Lambda LS OG 590

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Variable analysis

independent variables

Light stimuli intensity (effective photons per second)

dependent variables

Rate of single photon responses (bumps) from WT flies at normal Ca2+ conditions

control variables

Ca2+ conditions (normal)
Genotype (WT flies)

controls

Rate of single photon responses (bumps) from WT flies at normal Ca2+ conditions
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