Carboxysome Protein Identification by Mass Spectrometry

The shell samples collected from sucrose fractions were washed with PBS buffer and were treated for mass spectrometry analysis^{24 (link)}. Data-dependent LC−MS/MS analysis was performed on a QExactive quadrupole-Orbitrap mass spectrometer coupled to a Dionex Ultimate 3000 RSLC nano-liquid chromatograph (Thermo Fisher, UK) installed with Xcalibur software version 4.1. A Mascot Generic File, created by Progenesis QI (Version 3.0, Nonlinear Dynamics, Newcastle upon Tyne, UK), was searched against the H. neapolitanus carboxysome protein database from UniProt.

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Li T., Jiang Q., Huang J., Aitchison C.M., Huang F., Yang M., Dykes G.F., He H.L., Wang Q., Sprick R.S., Cooper A.I, & Liu L.N. (2020). Reprogramming bacterial protein organelles as a nanoreactor for hydrogen production. Nature Communications, 11, 5448.

Publication 2020

Dionex Ultimate 3000 RSLC

Buffer Generic Liquid chromatograph Mass spectrometry Ms ms Sucrose

Corresponding Organization : Ocean University of China

Other organizations : University of Liverpool, Central South University, Henan University

Top 5 similar protocols

Variable analysis

independent variables

Sucrose fractions

dependent variables

Mass spectrometry analysis

control variables

PBS buffer treatment
Dionex Ultimate 3000 RSLC nano-liquid chromatograph
QExactive quadrupole-Orbitrap mass spectrometer
Xcalibur software version 4.1
Progenesis QI (Version 3.0, Nonlinear Dynamics, Newcastle upon Tyne, UK)
H. neapolitanus carboxysome protein database from UniProt

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