Immunocytochemistry was performed using a rabbit polyclonal anti-P2Y12 antibody10 (link) (generated by Dr. H. Weiner, Harvard University, Cambridge, MA), a monoclonal anti-CD68 antibody (Dako, Burlington, Ontario, Canada; 1:100), and appropriate secondary antibodies. For human brain sections, slides were deparaffinized and antigen retrieval was performed using a 0.01 M sodium citrate buffer solution (pH 6.0) for 15 minutes in the microwave on high setting.
Protocol detail
Immunocytochemical Localization of P2Y12 and CD68
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Antibodies
Antigen
Brain
Buffer
Human
Immunocytochemistry
Microwave
Monoclonal antibody
Rabbit
Sodium citrate
Corresponding Organization :
Other organizations : Queen's University, Harvard University, Memorial University of Newfoundland, Montreal Neurological Institute and Hospital, McGill University, Brigham and Women's Hospital
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Variable analysis
independent variables
- Antibody type (rabbit polyclonal anti-P2Y12 antibody, monoclonal anti-CD68 antibody)
- Presence and localization of P2Y12 and CD68 proteins in human brain sections
- Deparaffinization of human brain sections
- Antigen retrieval using 0.01 M sodium citrate buffer solution (pH 6.0) for 15 minutes in the microwave on high setting
- Use of appropriate secondary antibodies
- Positive control: Not explicitly mentioned
- Negative control: Not explicitly mentioned
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