Visualizing Meningeal Lymphatic Vessels
Partial Protocol Preview
This section provides a glimpse into the protocol.
The remaining content is hidden due to licensing restrictions, but the full text is available at the following link:
Access Free Full Text.
Corresponding Organization : University of Virginia
Other organizations : Northwestern University
Variable analysis
- Route of administration (intravenous (i.v.) or into the cisterna magna (i.c.m.))
- Meningeal lymphatic vessel imaging
- Adult Prox1GFP, LckCre::tdTOMATOfl/fl mice
- Anesthesia with ketamine/xylazine intraperitoneal (i.p.) injection
- Furamidine dihydrochloride (125µg) injected i.v. prior to imaging
- Maintaining core temperature of mice at 37°C
- Imaging performed with Leica TCS SP8 multiphoton system with Chameleon Ultra II tunable Ti:sapphire laser
- Excitation wavelength of 880 nm (or 780nm for furamidine)
- Second harmonic signal (bone and meningeal signal) visualized using a 400/450 external detector
- Images obtained using a 25X water immersion objective with 0/95NA and external HyD non-descanned detectors
- Three-dimensional images data collected from x,y and z-planes
- Image analysis using Imaris software
- Qtracker655 vascular tracker (ThermoFisher - Qdot655)
- No negative control mentioned.
Annotations
Based on most similar protocols
As authors may omit details in methods from publication, our AI will look for missing critical information across the 5 most similar protocols.
About PubCompare
Our mission is to provide scientists with the largest repository of trustworthy protocols and intelligent analytical tools, thereby offering them extensive information to design robust protocols aimed at minimizing the risk of failures.
We believe that the most crucial aspect is to grant scientists access to a wide range of reliable sources and new useful tools that surpass human capabilities.
However, we trust in allowing scientists to determine how to construct their own protocols based on this information, as they are the experts in their field.
Ready to get started?
Sign up for free.
Registration takes 20 seconds.
Available from any computer
No download required
Revolutionizing how scientists
search and build protocols!